Preparation and pharmaceuticals of biphenyl benzamide-derived derivatives

ABSTRACT

The present invention provides a biphenyl benzamide-derived derivatives, which structure is selected from formula I or formula II 
     
       
         
         
             
             
         
       
     
     and the synthesis and the application thereof.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a Divisional of co-pending application Ser. No. 13/571,158, filed on Aug. 9, 2012, for which priority is claimed under 35 U.S.C. §120; and this application claims priority of Application No. 101113752 filed in Taiwan on Apr. 18, 2012 under 35 U.S.C. §119; the entire contents of all of which are hereby incorporated by reference.

FIELD OF THE INVENTION

The present invention is related to a pharmaceutical composition of biphenyl benzamide-derived derivatives, the synthesis and the application thereof, especially related to the application of treating inflammatory reaction, osteoporosis, osteoarthritis and cancer.

BACKGROUND OF THE INVENTION

In the bone regeneration process, once the balance of the bone remodeling is broken, the bone resorption of the osteoclasts is dominated over bone formation of the osteoblasts, the bone remodeling would be imbalanced. As a result, the osteocytes would decrease, osteopenia or bone mineral density decrease to induce lots of bone disease, such as osteoporosis, periodontitis or osteoarthritis.

The osteoclasts origins from hematopoietic precursor cells. Further, the Macrophage-Colony Stimulating Factor (M-CSF) secreted by osteoblast and Receptor Activator of Nuclear factor Kappa B Ligand (RANK. L) would combine with the c-Fms and RANK on the cell membrane of the osteoclast precursor cells, and induce the secretion of tartrate-resistant acid phosphatase (TRAP), integrin b3 expression, and actin ring formation, etc. These changes of protein activity and cell morphology would enhance the osteoclasts motility and help the osteoclasts adhere on the bone surface. On the other hand, the expression of cathepsin K matrix metalloproteinase-9 (MMP-9), dendritic cell-specific transmembrane protein (DC-STAMP), ATPase, H+ transporting lysosomal V0 subunit D2(ATP6V0D2)would induce the osteoclast precursor cells differentiate into a hung matured (diameter is 20-100 mm) multinucleated cells (MNCs) (containing 4-20 nucleus), which also have the bone resorption function.

The osteoblasts would secret M-CSF and RANKL, which induce the osteocytes growth and differentiation, and would also secret the osteoprotegerin (OPG). OPG would associate with RANKL to prevent the association of RANKL and RANK, so as to prevent the formation of osteoclasts and inhibit the formation of osteoclasts, to decrease the bone resorption; besides, OPG would involve in the osteoclast apoptosis.

SUMMARY OF THE INVENTION

For the purpose, the present invention provides a series of pharmaceutical compositions of biphenyl benzamide-derived derivatives, wherein the pharmaceutical compositions can be used for treating inflammatory reaction, cancer and preventing osteoporosis and osteoarthritis effectively.

The present invention provides a biphenyl benzamide-derived derivatives, which structure is selected from formula I or formula II:

The present invention provides a pharmaceutical composition of biphenyl benzamide-derived derivatives, which comprises:

(a) a structure is selected from formula I or formula II:

(b) a pharmaceutical acceptable salt and carrier of the biphenyl benzamide-derived derivatives.

Preferably, the R₁, R₂, R₃ and R₄ of the formula I or formula II can be selected from the group of H, halogen, CF₃, CN, CH and OCH₃.

Preferably, the pharmaceutical acceptable carrier is excipient, diluents, thickeners, filler, binder, disintegrants, lubricant, oil or non-oil base, surfactant, suspending agent, gelling agent, adjuvant, anti-corrosive agent, anti-oxidant, stabilizer, coloring agent or flavor.

Preferably, the salt is physiological acceptable salt of inorganic acid, inorganic base, organic acid or organic base.

Preferably, the composition is powder, granule, liquid, gel or cream.

Preferably, the composition is administrated through oral, transdermal, injection, or inhalational manner.

The present invention also provides a method for synthesis of the compound of formula I, which is synthesized by difunisal:

The present invention also provides a method for synthesis of the compound of formula II, which is synthesized by compound of formula I:

Preferably, the compound of formula I is synthesized by amine, tetrahydrofuran and an intermediate compound which is synthesized by difunisal, tetrahydrofuran and thionyl chloride.

Preferably, the compound of formula II is synthesized by compound of formula I, tetrahydrofuran/pyridine and methyl chloroformate.

Preferably, the pharmaceutical composition can be used as anti-inflammatory agent, osteoporosis therapeutics or osteoarthritis therapeutics.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1A shows the effects of different concentration of compound 4 and compound 16 on the osteoclast proliferation.

FIG. 1B shows the effects of different concentration of compound 4 and compound 16 on the bone resorption activity.

FIG. 2A shows the osteoclast differentiation of RAW264.7; RAW264.7 cells which were cultured without the compound 4 and compound 16 without the presence of RANKL.

FIG. 2B shows the osteoclast differentiation of RAW264.7; RAW264.7 cells which were cultured without the compound 4 and compound 16 in the presence of RANKL.

FIG. 2C shows the osteoclast differentiation of RAW264.7; RAW264.7 cells which were cultured with the compound 4 and compound 16 without the presence of RANKL.

FIG. 2D shows the osteoclast differentiation of RAW264.7; RAW264.7 cells which were cultured with the compound 4 and compound 16 in the presence of RANKL.

FIG. 3A is the Mayer's hematoxylin staining shows the effects of compound 4 and compound 16 on osteoclast differentiation of RAW264.7; RAW264.7 cells were cultured without compound 4 and compound 16 and without the presence of RANKL.

FIG. 3B is the Mayer's hematoxylin staining shows the effects of compound 4 and compound 16 on osteoclast differentiation of RAW264.7; RAW264.7 cells were cultured without compound 4 and compound 16 and in the presence of RANKL.

FIG. 3C is the Mayer's hematoxylin staining shows the effects of compound 4 and compound 16 on osteoclast differentiation of RAW264.7; RAW264.7 cells were treated with compound 4 and compound 16 and without the presence of RANKL.

FIG. 3D is the Mayer's hematoxylin staining shows the effects of compound 4 and compound 16 on osteoclast differentiation of RAW264.7; RAW264.7 cells were treated with compound 4 and compound 16 and in the presence of RANKL.

FIG. 4 shows the IC₅₀ value of the compounds of the present invention on anti-inflammatory reaction.

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides a series of pharmaceutical compositions of biphenyl benzamide-derived derivatives, which can be used to inhibit the osteoclast growth so as to decrease the bone resorption and prevent the osteoporosis.

The structure of a biphenyl benzamide-derived derivative is selected from formula I or formula II:

The pharmaceutical composition of the present invention comprises, but not limited to,

(a) a structure is selected from formula I or formula II:

(b) a pharmaceutical acceptable salt and carrier of the biphenyl benzamide-derived derivatives.

In the best embodiment, the R₁, R₂, R₃ and R₄ of the formula I or formula II can be selected from the group consisting of H, halogen, CF₃, CN, CH and OCH₃.

The present invention also provides a method for synthesis of the compound of formula I, which is synthesized by difunisal:

The present invention also provides a method for synthesis of the compound of formula II, which is synthesized by compound of formula I:

In the best embodiment, the compound of formula I is synthesized by amine, tetrahydrofuran and an intermediate compound which is synthesized by difunisal, tetrahydrofuran and thionyl chloride.

In the best embodiment, the compound of formula II is synthesized by compound of formula I, tetrahydrofuran/pyridine and methyl chloroformate.

The present invention provides a method for treating inflammation, osteoporosis, or osteoarthritis by said pharmaceutical composition of the biphenyl benzamide-derived derivatives.

In the best embodiment, the pharmaceutical acceptable carrier is excipient, diluents, thickeners, filler, binder, disintegrants, lubricant, oil or non-oil base, surfactant, suspending agent, gelling agent, adjuvant, anti-corrosive agent, anti-oxidant, stabilizer, coloring agent or flavor.

In the best embodiment, the excipient can be, but not limited to, diluents, filler, binder, disintegrants, etc. Wherein the excipient can be, but not limited to, microcrystalline cellulose, polyvinylpyrrolidone (PVP), modified starches, sodium starch glycolate, gelatinized starches, polyethylene glycol (PEG), polyvinyl alcohol, hydroxypropyl cellulose, methylcellulose, hydroxymethyl cellulose, hydroxypropyl methylcellulose.

In the best embodiment, the salt can be physiological acceptable salt of inorganic acid, inorganic base, organic acid or organic base. Besides, the pharmaceutical composition is powder, granule, liquid, gel or cream. In addition, the pharmaceutical composition is administrated through oral, transdermal, injection, or inhalational manner.

As mentioned above, the various substitutes would be listed in Table 1, and the synthesis method of these biphenyl benzamide-derived derivatives would be further disclosed in the embodiments.

The embodiments of the present invention are listed in Table 2, this table shows the structure of the biphenyl benzamide-derived derivatives of the present invention.

TABLE 1 the R₁ to R₄ of the compounds of the present invention Compounds R₁ R₂ R₃ R₄ compound 1 and compound 13 F H Cl H compound 2 and compound 14 F H F H compound 3 and compound 15 H F F H compound 4 and compound 16 F H H F compound 5 and compound 17 CF₃ H H H compound 6 and compound 18 H CF₃ H H compound 7 and compound 19 H H CF₃ H compound 8 and compound 20 H CH H H compound 9 and compound 21 H CN H H compound 10 and compound 22 H H H CN compound 11 and compound 23 H OCH₃ OCH₃ H compound 12 and compound 24 H OCH₃ H H

TABLE 2 The structure of series of pharmaceutical compositions of biphenyl benzamide-derived derivatives.

Com- pound 1

Com- pound 2

com- pound 3

Com- pound 4

Com- pound 5

Com- pound 6

Com- pound 7

Com- pound 8

Com- pound 9

Com- pound 10

Com- pound 11

Com- pound 12

Com- pound 13

Com- pound 14

Com- pound 15

Com- pound 16

Com- pound 17

Com- pound 18

Com- pound 19

Com- pound 20

Com- pound 21

Com- pound 22

Com- pound 23

Com- pound 24

The synthesis method and the details of the compounds 1 to 24 of the present invention is described as embodiments 1 to 24:

EXAMPLE 1 N-(4-chloro-2-fluorophenyl)-2′,4′-difluoro-4-hydroxybiphenyl-3-carboxamide (compound 1)

The synthesis method of compound 1:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 4-chloro-2-fluoroaniline (0.5 mL, 4 mmol) in anhydrous tetrahydrofuran (30 ml) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 1.

Yield: 30%. Mp: 245-246° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.12 (d, J=6.0 Hz, 1H, Ar—H₅), 7.19 (td, J=9.0, 1.8 Hz, 1H, Ar—H_(6′)), 7.31-7.40 (m, 2H, Ar—H_(5, 6″)), 7.55-7.64 (m, 3H, Ar—H_(6, 3′, 5″)), 8.14 (t, J=1.5 Hz, 1H, Ar—H₂), 8.24 (t, J=8.7 Hz, 1H, Ar—H_(3″)), 10.77 (s, NH), 12.19 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 377.0430 (C₁₉H₁₁ClF₃NO₂ ⁺); found, 377.33.

EXAMPLE 2 N-(2,4-difluorophenyl)-2′,4′-difluoro-4-hydroxybiphenyl-3-carboxamide (compound 2)

The synthesis method of compound 2:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 2,4-difluoroaniline (0.4 mL, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 2.

Yield: 42%. Mp: 233-234° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.10-7.23 (m, 3H Ar—H_(5, 6′, 6″)), 7.33-7.45 (m, 2H, Ar—H_(5′, 5″)), 7.55-7.64 (m, 2H, Ar—H_(6, 3′)), 8.06-8.15 (m, 2H, Ar—H_(2, 3″)), 10.64 (s, 1H, NH), 12.16 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 361.0726 (C₁₉H₁₁F₄NO₂ ⁺); found, 361.0730.

EXAMPLE 3 N-(3,4-difluorophenyl)-2,4′-difluoro-4-hydroxy-[1,1′-biphenyl]-3-carboxamide (compound 3)

The synthesis method of compound 3:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 3,4-difluoroaniline (0.4 mL, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 3.

Yield: 61%. Mp: 231-232° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7. 09 (d, J=8.7 Hz, 1H, Ar—H₅), 7.20 (td, J=8.4, 3 Hz, 1H, Ar—H_(6′)), 7.45-7.49 (m, 3H, Ar—H_(3′, 5′, 6″)), 7.56-7.64 (m, 2H, Ar—H_(6, 5″)), 7.87-7.93 (m, 1H, Ar—H_(6″)), 8.01 (t, J=0.9 Hz, 1H, 10.55 (s, 1H, NH), 11.70 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 361.0726 (C₁₉H₁₁F₄NO₂ ⁺); found, 361.0724.

EXAMPLE 4 N-(2,5-difluorophenyl)-2′,4′-difluoro-4-hydroxy-[1,1′-biphenyl]-3-carboxamide (compound 4)

The synthesis method of compound 4:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 2,5-difluoroaniline (0.4 mL, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 4.

Yield: 37%. Mp: 213-214° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 6.99-7.05 (m, 1H, Ar—H_(4″)), 7.12-7.22 (m, 2H, Ar—H_(5, 6′)), 7.33-7.44 (m, 2H, Ar—H_(5′, 3″)), 7.55-7.65 (m, 2H, Ar—H_(6, 3′)), 8.14-8.23 (m, 2H, Ar—H_(2, 6″)), 10.89 (s, 1H, NH), 12.22 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 361.0726 (C₁₉H₁₁F₄NO₂ ⁺); found, 361.0731.

EXAMPLE 5 2′,4′-difluoro-4-hydroxy-2-(trifluoromethyl)phenyl)-[1,1′-biphenyl]-3-carboxamide (compound 5)

The synthesis method of compound 5:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 2-(trifluoromethyl)aniline (0.5 mL, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 5.

Yield: 36%. Mp: 180-181° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.11-7.22 (m, 2H, Ar—H_(5, 6′)), 7.32-7.43 (m, 2H, Ar—H_(5′, 3″)), 7.54-7.65 (m, 2H, Ar—H_(6, 3′)), 7.70-7.78 (m, 2H, Ar—H_(4″, 5″)), 8.18-8.20 (m, 2H, Ar—H_(2, 6″)), 10.81(s, 1H, NH), 12.19 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 393.0788 (C₂₀H₁₂F₅NO₂ ⁺); found, 393.0784.

EXAMPLE 6 2′,4′-difluoro-4-hydroxy-N-(3-(trifluoromethyl)phenyl)[1,1′-biphenyl]-3-carboxamide (compound 6)

The synthesis method of compound 6:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 3-(trifluoromethyl)aniline (0.5 mL, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 6.

Yield: 32%. Mp: 202-203° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.10 (d, J=8.7 Hz, 1H, Ar—H₅), 7.20 (td, J=9, 2.7 Hz, 1H, Ar—H_(6′)), 7.37 (td, J=11.1, 2.4 Hz, 1H, Ar—H₅), 7.49 (d, J=7.8 Hz, 1H, Ar—H_(6″)), 7.57-7.65 (m, 3H, Ar—H_(3′, 4″, 5″)), 7.95 (d, J=8.1 Hz, 1H, Ar—H₆), 8.05 (t, J=1.2 Hz, 1H, Ar—H₂), 8.21 (s, 1H, Ar—H_(2″)), 10.66 (s, 1H, NH), 11.70 (s, 1H, OH). HRMS (ET) m/z: calcd [M]⁺, 393.0788 (C₂₀H₁₂F₅NO₂ ⁺); found, 393.0787.

EXAMPLE 7 2′,4′-difluoro-4-hydroxy-N-(4-(trifluoromethyl)phenyl)-[1,1′-biphenyl]-3-carboxamide (compound 7)

The synthesis method of compound 7:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 4-(trifluoromethyl)aniline (0.5 mL, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 7.

Yield: 44%. Mp: 227-228° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.10 (d, J=8.4 Hz, 1H, Ar—H₅), 7.18 (td, J=13.7, 1.5 Hz, 1H, Ar—H_(6′)), 7.36 (td, J=9.3, 2.7 Hz, 1H, Ar—H_(5′)), 7.74 (d, J=9.0 Hz, 2H, Ar—H_(3″, 5″)), 7.95 (d, J=8.4 Hz, 2H, Ar—H_(2″, 6″)), 8.03 (s, 1H, Ar—H₂), 10.67 (s, 1H, NH), 11.66 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 393.0788 (C₂₀H₁₂F₅NO₂ ⁺); found, 393.0791.

EXAMPLE 8 N-(3-ethynylphenyl)-2′,4′-difluoro-4-hydroxy-[1,1′-biphenyl]-3-carboxamide (compound 8)

The synthesis method of compound 8:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 3-ethynylaniline (0.45 mL, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 8.

Yield: 38%. Mp: 215-216° C. (EtOH). ¹H-NMR. (300 MHz, DMSO-d₆): δ ppm 7. 09 (d, J=8.7 Hz, 1H, Ar—H₅), 7.17-7.26 (m, 2H, Ar—H_(4″, 6′)), 7.33-7.41 (m, 2H, Ar—H_(5′, 5″)), 7.57-7.65 (m, 2H, Ar—H_(6, 3′)), 7.70-7.73 (m, 1H, Ar—H_(6″)), 7.89 (t, J=1.8 Hz, 1H, Ar—H₂), 8.05 (t, J=1.2 Hz, 1H, Ar—H_(2″)), 10.48 (s, 1H, NH), 11.79 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 348.0836 (C₂₁H₁₂F₂NO₂ ⁺); found, 348.0835.

EXAMPLE 9 N-(3-cyanophenyl)-2′,4′-difluoro-4-hydroxy-[1,1′-biphenyl]-3-carboxamide (compound 9)

The synthesis method of compound 9:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 3-aminobenzonitrile (0.47 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 9.

Yield: 51%. Mp: 229-230° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.10 (d, J=8.4 Hz, 1H, Ar—H₅), 7.16-7.22 (m, 1H, Ar—H_(6′)), 7.35 (td, J=10.35, 2.7 Hz, 1H, Ar—H_(5′)) 7.55-7.63 (m, 4H, Ar—H_(6, 3′, 4″, 5″)), 7.96-8.00 (m, 1H, Ar—H_(6″)), 8.02 (t, J=0.9 Hz, 1H, Ar—H₂), 8.21 (t, J=0.9 Hz, 1H, Ar—H_(2″)), 10.63 (s, 1H, NH), 11.66 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 350.0867 (C₂₀H₁₂F₂N₂O₂ ⁺); found, 350.0857.

EXAMPLE 10 N-(4-cyanophenyl)-2′,4′-difluoro-4-hydroxy-[1,1′-biphenyl]-3-carboxamide (compound 10)

The synthesis method of compound 10:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 4-aminobenzonitrile (0.47 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 10.

Yield: 54%. Mp: 189-190° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.19 (td, J=8.4, 0.9 Hz, 1H, Ar—H_(6′)), 7.35 (td, J=10.2, 2.4 Hz, 1H, Ar—H_(5′)), 7.55-7.63 (m, 2H, Ar—H_(6, 3′)), 7.81-7.84 (m, 2H, Ar—H_(3″, 5″)), 7.91-7.94 (m, 1H, Ar—H_(2″, 6″)), 7.98 (t, J=1.2 Hz, 1H, Ar—H₂), 10.70 (s, 1H, NH), 11.55 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 350.0867 (C₂₀H₁₂F₂N₂O₂ ⁺); found, 350.0872.

EXAMPLE 11 N-(3,4-dimethoxyphenyl)-2′,4′-difluoro-4-hydroxy-[1,1′-biphenyl]-3-carboxamid (compound 11)

The synthesis method of compound 11:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 3,4-dimethoxyaniline (0.61 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 11.

Yield: 34%. Mp: 186-187° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 3.75 (s, 3H, OCH₃), 3.76 (s, 3H, OCH₃), 6.95 (d, J=9 Hz, 1H, Ar—H_(2″)), 7.07 (d, J=6.6 Hz, 1H, Ar—H₅), 7.17-7.25 (m, 2H, Ar—H_(6′, 5″)), 7.31-7.39 (m, 2H, Ar—H_(5′, 6″)), 7.56-7.66 (m, 2H, Ar—H_(6, 3′)), 8.11 (s, 1H, Ar—H₂), 10.33 (s, 1H, NH), 12.12 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 385.1126 (C₂₁H₁₇F₂NO₄ ⁺); found, 385.1124.

EXAMPLE 12 2′,4′-difluoro-4-hydroxy-N-(3-methoxyphenyl)-[1,1′-biphenyl]-3-carboxamide (compound 12)

The synthesis method of compound 12:

Thionyl chloride (1 mL, 14 mmol) was added to disfunisal (0.9 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL), and the mixture was refluxed under nitrogen atmosphere for 8 h. After cooling to room temperature, the mixture was steamed to give the intermediate by Dean-Stark apparatus, which residue was used directly in the next step. Freshly prepared intermediate was directly reacted with 3-methoxyaniline (0.46 g, 4 mmol) in anhydrous tetrahydrofuran (30 mL) for 14 h. After removal of tetrahydrofuran, the reaction mixture was washed with ethyl acetate/hexane and the crude product was extracted in ethyl acetate. The organic layer was collect and dried over anhydrous Na₂SO₄, and then the solvent was evaporated. The crude product was washed and purified by crystallization from hot ethanol to afford compound 12.

Yield: 32%. Mp: 187-188° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 3.76 (s, 3H, OCH₃), 6.70-6.74 (m, 1H, Ar—H_(5″)), 7.08 (d, J=8.4 Hz, 1H, Ar—H₅), 7.16-7.39 (m, 4H, Ar—H_(6′, 2″, 4″, 6″)) 7.57-7.65 (m, 2H, Ar—H_(6, 3′)), 8.07 (s, 1H, Ar—H₂), 10.39 (s, 1H, NH), 11.90 (s, 1H, OH). HRMS (EI) m/z: calcd [M]⁺, 355.100 (C₂₀H₁₅F₂NO₃ ⁺); found, 355.1014.

The compounds 13-24 are prepared by the compounds 1-12 in the present invention. The synthesis methods are showed as example 13-24:

EXAMPLE 13 3-(4-chloro-2-fluorophenyl)-6-(2,4-difluorophenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 13)

The synthesis method of compound 13:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 1 (1.5 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 2.5 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 13.

Yield: 11%. Mp: 176-177° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.23 (td, J=8.4, 2.5 Hz, 1H, Ar—H_(6′)), 7.23 (td, J=10.4, 2.7 Hz, 1H, Ar—H_(5′)), 7.48-7.52 (m, 1H, Ar—H_(6″)), 7.63-7.75 (m, 4H, Ar—H_(5, 3′, 3″, 5″)), 8.05 (dt, J=8.4, 1.5 Hz, 1H, Ar—H₆), 8.10 (t, J=1.8 Hz, 1H, Ar—H₂), HRMS (EI) m/z: calcd [M]⁺, 403.0223 (C₂₀H₉ClF₃NO₃ ⁺); found, 403.0229.

EXAMPLE 14 3,6-bis(2,4-difluorophenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 14)

The synthesis method of compound 14:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 2 (1.44 g, 4 mmol) in dry anhydrous tetrahydrofuranipyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 14.

Yield: 61%. Mp: 155-156° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.20-7.33 (m, 2H, 7.42 (td, J=9.7, 1.5 Hz, Ar—H_(5′)), 7.54 (td, J=9.7, 2.1 Hz, 1H, Ar—H_(6′)), 7.65-7.74 (m, 3H, Ar—H_(5, 3′, 3″)), 8.03-8.10 (m, 2H, Ar—H_(2, 6)). HRMS (EI) m/z: calcd [M]⁺, 387.0519 (C₂₀H₉F₄NO₃ ⁺); found, 387.0518.

EXAMPLE 15 6-(2,4-difluorophenyl)-3-3,4-difluorophenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 15)

The synthesis method of compound 15:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 3 (1.44 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The etude product was purified by crystallization from hot ethanol to afford compound 15.

Yield: 25%. Mp: 193-194° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.20-7.23 (m, 1H, Ar—H_(6′)), 7.34-7.45 (m, 2H, Ar—H_(5′, 2″)), 7.57-7.73 (m, 4H, Ar—H_(5, 3′, 5″, 6″)), 8.03-8.04 (m, 1H, Ar—H₆), 8.08 (t, J=1.8 Hz, 1H, Ar—H₂). HRMS (EI) m/z: calcd [M]⁺, 387.0519 (C₂₀H₉F₄NO₃ ⁺); found, 387.0522.

EXAMPLE 16 6-(2,4-difluorophenyl)-3-(2,5-difluorophenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 16)

The synthesis method of compound 16:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 4 (1.44 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 16.

Yield: 15%. Mp: 144-145° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.23 (td, J=8.4, 2.4 Hz, 1H, Ar—H_(6′)), 7.37-7.58 (m, 4H, Ar—H_(5′, 3″, 4″, 6″)), 7.65-7.73 (m, 2H, Ar—H_(5, 3′)), 8.03-8.11 (m, 2H, Ar—H_(2, 6)). HRMS (EI) m/z: calcd [M]⁺, 387.0519 (C₂₀H₉F₄NO₃ ⁺); found, 387.0516.

EXAMPLE 17 6-(2,4-difluorophenyl)-3-(2-(trifluoromethy)phenyl)-2H-benzo[e][oxazine-2,4(3H)-dione (compound 17)

The synthesis method of compound 17:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 5 (1.56 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 17.

Yield: 31%. Mp: 200-201° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.23 (td, J=9.3, 2.4 Hz, 1H, Ar—H_(6″)), 7.42 (td, J=10.2, 2.4 Hz, 1H, Ar—H_(5′)), 7.68-7.82 (m, 4H, Ar—H_(5, 3′, 3″, 6″)), 7.89-7.95 (m, 2H, Ar—H_(4″, 5″)), 8.05-8.11 (m, 2H, Ar—H_(2, 6)). HRMS (EI) m/z: calcd [M]⁺, 419.0581 (C₂₁H₁₀F₅NO₃ ⁺); found, 419.0586.

EXAMPLE 18 6-(2,4-difluorophenyl)-3-(3-(trifluoromethyl)phenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 18)

The synthesis method of compound 18:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 6 (1.56 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stifling at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 18.

Yield: 13%, Mp: 165-166° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.24 (td, J=7.5, 2.1 Hz, 1H, Ar—H_(6′)), 7.42 (td, J=10.1, 2.7 Hz, 1H, Ar—H_(5′)), 7.64-7.73 (m, 2H, Ar—H_(5, 3′)), 7.78-7.80 (m, 2H, Ar—H_(4″, 6″)), 7.85-7.88 (m, 1H, Ar—H_(5″)), 7.91 (s, 1H, Ar—H_(2″)), 8.03 (dt, J=9.3, 1.5 Hz, 1H, Ar—H₆), 8.08 (t, J=1.8 Hz, 1H, Ar—H₂). HRMS (EI) m/z: calcd [M]⁺, 419.0581. (C₂₁H₁₀F₅NO₃ ⁺); found, 419.0590.

EXAMPLE 19 6-(2,4-difluorophenyl)-3-(4-(trifluoromethyl)phenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 19)

The synthesis method of compound 19:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 7 (1.56 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 19.

Yield: 11%. Mp: 209-210° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.24 (td, J=9, 1.2 Hz, 1H, Ar—H_(6′)), 7.42 (td, J=10.2, 2.4 Hz, 1H, Ar—H_(5′)), 7.63-7.73 (m, 4H, Ar—H_(3, 5, 3″, 5″)), 7.93 (d, J=8.4 Hz, 2H, Ar—H_(2″, 6″)), 8.03 (dt, J=8.7, 2.1 Hz, 1H, Ar—H₆), 8.08 (t, J=1.5 Hz, 1H, Ar—H₂). HRMS (EI) m/z: calcd [M]⁺, 419.0581 (C₂₁H₁₀F₅NO₃ ⁺); found, 419.0584.

EXAMPLE 20 6-(2,4-difluorophenyl)-3-(3-ethynylphenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 20)

The synthesis method of compound 20:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 8 (1.39 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 20.

Yield: 14%. Mp: 195-196° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.23 (td, J=8.3, 2.7 Hz, 1H, Ar—H_(6′)), 7.42 (td, J=10.2, 2.4 Hz, 1H, Ar—H_(5′)), 7.48-7.73 (m, 6H, Ar—H_(5, 3′, 2″, 4″, 5″, 6″)), 7.99-8.04 (m, 1H, Ar—H₆), 8.07 (t, J=1.8 Hz, 1H, Ar—H₂). HRMS (EI) m/z: calcd [M]⁺, 375.0707 (C₂₁H₁₁F₂NO₃ ⁺); found, 375.0708.

EXAMPLE 21 3-(6-(2,4-difluorophenyl)-2,4-dioxo-2H-benzo[e][1,3]oxazin-3(4H)-yl)benzonitrile (compound 21)

The synthesis method of compound 21:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 9 (1.4 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% 1HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 21.

Yield: 49%. Mp: 209-210° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.21-7.27 (m, 1H, Ar—H_(6′)), 7.42 (td, J=10.5, 2.4 Hz, 1H, Ar—H_(5′)), 7.64-7.86 (m, 5H, Ar—H_(5, 3′, 4″, 5″, 6″)) 7.96-8.05 (m, 2H, Ar—H_(6, 2″)), 8.09 (t, J=1.8 Hz, 1H, Ar—H₂). HRMS (EI) m/z: calcd [M]⁺, 376.0659 (C₂₁H₁₀F₂N₂O₃ ⁺); found, 375.0622.

EXAMPLE 22 4-(6-(2,4-difluorophenyl)-2,4-dioxo-2H-benzo[e][1,3]oxazin-3(4H)-yl)benzonitrile (compound 22)

The synthesis method of compound 22:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 10 (1.4 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 22.

Yield: 22%. Mp: 203-204° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 7.24 (td, J=8.7, 0.9 Hz, 1H, Ar—H_(6′)), 7.42 (td, J=10.1, 2.7 Hz, 1H, Ar—H_(5′)), 7.63-7.73 (m, 4H, Ar—H_(5, 3′, 3″, 4″)), 8.01-8.08 (m, 4H, Ar—H_(2, 6, 2″, 6″)). HRMS (EI) m/z: calcd [M]⁺, 376.0659 (C₂₁H₁₀F₂N₂O₃ ⁺); found, 375.0655.

EXAMPLE 23 6-(2,4-difluorophenyl)-3-(3,4-dimethoxyphenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 23)

The synthesis method of compound 23:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 11 (1.54 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0° C. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 23.

Yield: 43%. Mp: 198-199° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 3.72 (s, 3H, OCH₃), 3.81 (s, 3H, OCH₃), 6.96 (dd, J=8.5, 2.1 Hz, 1H Ar—H_(5″)), 7.04-7.09 (m, 2H, Ar—H_(2″, 6″)), 7.42 (td, J=8.3, 2.7 Hz, 1H, Ar—H_(6′)), 7.61 (d, J=8.4 Hz, 1H, Ar—H₅), 7.65-7.73 (m, 1H, Ar—H_(3′)), 7.98-8.02 (m, 1H, Ar—H₅), 8.07 (t, J=1.5 Hz, 1H, Ar—H₂). HRMS (EI) m/z: calcd [M]⁺, 411.0918 (C₂₂H₁₅F₂NO₅ ⁺); found, 411.0917.

EXAMPLE 24 6-(2,4-difluorophenyl)-3 -(3 -methoxyphenyl)-2H-benzo[e][1,3]oxazine-2,4(3H)-dione (compound 24)

The synthesis method of compound 24:

A solution of methyl chloroformate (1.2 mL, 12 mmol) was added drop wised to a stirred solution of compound 12 (1.42 g, 4 mmol) in dry anhydrous tetrahydrofuran/pyridine (30 mL) at 0□. The mixture was refluxed for 3 h. After 10 h stirring at room temperature, the pH value of the mixture was adjusted to pH=6 by 5% HCl_((aq)). The mixture was cooled to obtain crystalline compound on an ice bath for 2-3 h. After cooling, precipitated crystals were filtered off and washed with diluted HCl and water. The crude product was purified by crystallization from hot ethanol to afford compound 24.

Yield: 12%. Mp: 160-161° C. (EtOH). ¹H-NMR (300 MHz, DMSO-d₆): δ ppm 3.77 (s, 3H, OCH₃), 6.99-7.07 (m, 2H, Ar—H_(4″, 5″)), 7.20-7.27 (m, 1H, Ar—H_(6′)), 7.38-7.45 (m, 2H, Ar—H_(5′, 6′)), 7.23-7.60 (m, 2H, Ar—H_(5, 3′)), 7.98-8.03 (m, 1H, Ar—H₆), 8.07 (t, J=1.8 Hz, 1H, Ar—H₂). HRMS (EI) m/z: calcd [M]⁺, 381.0813 (C₂₁H₁₃F₂NO₄ ⁺); found, 381.0806.

The following examples is the activity of compounds 1 to 24 of the examples 1 to 24:

EXAMPLE 25 The Pharmacological Activity Test and Result

The compounds 1 to 24 of the present invention is tested by three pharmacological activity tests as follows: (1) MTT assay to test the RAW264.7 cell (murine monocyte/macrophage cell line) viability; (2) Tartrate-resistant acid phosphatase (TRAP) staining and activity analysis to investigate the cell differentiation activity; (3) The activity test of bone resorption (Pit formation assay). By these testes, the biphenyl benzamide-derived derivatives containing pharmaceutical composition, which comprises the formula I compound, were proved to perform effects of inhibition on osteoclastogenesis.

The synthesized difunisal derivatives, compounds 1 to 24, were pharmacologically tested to be identified the relationship between their structure and activity. Cell viability was measured by MTT assay in RAW 264.7 cells upon treatment with compounds at 5 μM. CC₅₀ is the cytotoxic concentration that produces 50% (CC₅₀) cell death. As shown in Table 3, the cell viability was more than 90% upon treatment with compounds at 5 μM. The multinucleated osteoclasts could be formed by RANKL-induced macrophage cell line RAW267. RAW 264.7 cells were cultured with the indicated in the presence of drug and RANKL (100 ng/mL). Numbers of TRAP-positive (TRAP⁺) multinucleated cells (MNCs) were counted in the presence of RANKL to identify the relationship between osteoclast and bone formation. To avoid the effect of cell viability, the concentration of synthesized compounds was 5 μM, and the result was showed in Table 4. Some of the derivatives perform more than 50% inhibitory activity on RANKL-induced osteoclast differentiation, wherein the compound 4, compound 9, compound 16 and compound 18 perform 67.32±4.38%, 71.32±3.39%, 86.84±1.74% and 62.92±2.65% inhibitory activity of TRAP-positive (TRAP⁺) multinucleated cells (MNCs) formation. The compound 16 performed the best inhibitory effect in the TRAP staining. The compound 16 was a derivate from compound 4 and its structure was related to compound 4. Therefore, the compound 4 and 16 were performed by TRAP assay to evaluate the effect on osteoclast differentiation by different concentration of compounds. As shown in FIG. 1A, compound 4 and 16 performed TRAP inhibitory activity, the IC₅₀ were 3.89 μM and <2.5 μM, respectively. According to above result, it can be speculated that: (1) Due to different substitutes on benzene ring of difunisal, the inhibitory ability of RANKL-induced osteoclast differentiation would be different. (2) Due to the electron-withdrawing group substitutes of R₁ and R₄ on benzene ring, or electron-withdrawing group substitutes derivatives of R2 on benzene ring, such as compound 4. (R₁═F and R₄═F), compound 6 (R₂═CF₃), compound 9 (R₂═CN) and compound 16 (R₁═F and R₄═F), (3) compound 4 (R₁═F and R₄═F) and compound 6 (R₂═CF₃), a cyclic derivate compound 16 (R₁═F and R₄═F) and compound 18 (R₂═CF₃), the inhibitory activity would be stronger. FIG. 2 showed the effect on RAW264.7 cell differentiation by compound 4 and 16 with or without the presence of RANKL.

EXAMPLE 26 Effect on Inhibition Resorption of Osteoclast by Compounds 1˜24

By using pit formation assay, compounds 4 and 16 have effect on inhibition of resorption of osteoclast. RAW264.7 cells were cultured without compounds (compound 4 or compound 16, 5 μM) or treated with compounds (compound 4 or compound 16, 5 μM) in 24 well dentine slices plates in the presence of RANKL after 4 days, to identify the ability of resorption of osteoclast by observing the resorption pits formation. As shown in FIG. 1B, compared to the cell cultured without drug in the presence of RNAKL, the resorbed area of the cell cultured in compound 4 or 16 was only 23.11±2.25% and 20.11±1.51% respectively. It was obvious that compound 4 or 16 could inhibit the RANKL-induced resorption of osteoclast (FIG. 3A to D).

TABLE 3 Cell viability and CC₅₀ values of synthesized compounds in RAW 264.7 cells RAW 264.7 cells Compound Cell viability (%) CC₅₀ 1 77.54 ± 1.17 17.85 2 109.62 ± 3.37  >20 3 78.42 ± 3.79 14.59 4 102.46 ± 1.10  14.56 5 99.37 ± 3.71 >20 6 96.73 ± 1.18 14.35 7 85.22 ± 3.83 10.38 8 62.95 ± 0.85 >20 9 72.15 ± 0.36 12.27 10 96.24 ± 2.29 14.39 11 98.87 ± 2.06 >20 12 104.90 ± 2.62  >20 13 96.22 ± 0.53 >20 14 96.49 ± 1.86 12.73 15 72.15 ± 0.43 9.96 16 86.60 ± 5.69 10.81 17 95.53 ± 3.86 15.13 18 100.13 ± 0.94  12.98 19 103.01 ± 2.51  15.48 20 88.38 ± 4.65 11.01 21 102.79 ± 1.93  14.86 22 108.33 ± 1.10  >20 23 103.05 ± 4.03  >20 24 105.53 ± 4.7  >20 Diflunisal 103.82 ± 1.78  >20

TABLE 4 Effects of Inhibitory osteoclast differentiation by compounds 1 to 24 in RAW 264.7 cells RAW 264.7cell Compound Inhibition of MNCs (%)  1 44.30 ± 7.93  2 32.24 ± 5.58  3 47.37 ± 5.58  4 67.32 ± 4.38  5 42.76 ± 6.51  6 59.21 ± 2.28  7 51.54 ± 6.25  8 40.57 ± 2.01  9 71.32 ± 3.39 10 13.87 ± 1.23 11 51.93 ± 6.50 12 40.79 ± 2.65 13 19.41 ± 1.40 14 42.32 ± 6.62 15 17.35 ± 7.49 16 86.84 ± 1.74 17 31.58 ± 4.00 18 62.92 ± 2.65 19 16.85 ± 4.61 20 57.46 ± 3.25 21 33.99 ± 6.98 22 29.44 ± 5.48 23 21.20 ± 2.49 24 30.75 ± 5.77 Diflunisal 23.91 ± 1.88

EXAMPLE 27 The Anti-Inflammatory Pharmaceutical Application of Compounds 1˜24 in the Present Invention

The synthesized 24 biphenyl benzamide-derived derivatives drug, compounds 1 to 24, can be used as anti-osteoporosis agent, also can be a potential anti-inflammatory agent. After modified, most of the compounds performed stronger anti-inflammatory effect than difunisal of original NSAID (Nonsteroidal anti-inflammatory drugs).

Isolation and Culture of Porcine Chondrocytes

Porcine cartilage was obtained from the hind leg joints of pigs. The synthesis of chondrocytes from cartilage was performed according to our previous report. After enzymatic digestion of articular cartilage with 2 mg/mL protease in serum-free Dulbecco's modified Eagle's medium (DMEM)/antibiotics, the specimens were then digested overnight with 2 mg/mL collagenase I and 0.9 mg/ml, hyaluronidase in DMEM containing 10% fetal bovine serum (FBS). The cells were collected, passed through a cell strainer (Becton Dickinson, Mountain View, Calif., USA), and cultured in DMEM containing 10% PBS and antibiotics for 3-4 days before use.

Cytotoxicity Assay by Lactate Dehydrogenase (LDH) Leakage

The measurement of the concentrations of the released lactate dehydrogenase (LDH), as an indicator of damage to the plasma membrane, was performed according to the manufacturer's instructions (Roche, Indianapolis, Ind., USA). The percent cytotoxicity was calculated as ([sample value−medium control]/high control−medium control])×100. Individual sample values were the averages of the absorbance values in treated culture supernatants after subtraction of the absorbance values in background control in triplicate. Similarly, the average absorbance values of untreated cell culture supernatants, used as the medium control, were calculated. Equal amount of cells treated with 1% Triton X-100 was taken as the high control.

Measurement of NO Concentrations

The measurement of NO release was reflected by determination of its stable end product, nitrite, in supernatants. The Griess reaction was performed with the concentrations of nitrite measured by a spectrophotometer. In brief, an aliquot (100 μL) of culture supernatant was incubated with 50 μL of 0.1% sulfanilamide in 5% phosphoric acid and 50 μL of 0.1% N-1-naphthyl-ethylenediamine dihydrochloride. After 10 min of incubation at room temperature, the absorbance was measured at 550 nm wavelength with a plate reader (recall, Grodig, Australia). The result was showed in Table 5 and FIG. 4. By viewing the IC₅₀ values of synthesized compounds on NO production, the compound 18 (IC50=0.55) performed best effect on inflammatory factor—NO inhibition.

TABLE 5 The anti-inflammatory IC₅₀ (μM) a value of compounds 1 to 24 in the present invention Compound IC₅₀ (μM)^(a) compound 1 7.94 compound 2 0.95 compound 3 0.79 compound 4 0.71 compound 5 >10 compound 6 0.71 compound 7 0.64 compound 8 2.45 compound 9 >10 compound 10 >10 compound 11 1.5 compound 12 2.24 compound 13 1.08 compound 14 1.26 compound 15 0.61 compound 16 1.15 compound 17 >10 compound 18 0.55 compound 19 0.75 compound 20 1.63 compound 21 >10 compound 22 0.55 compound 23 0.47 compound 24 1.95 Diflunisal >10

As mentioned above, the present invention provides a series of pharmaceutical compositions of biphenyl benzamide-derived derivatives, the pharmaceutical acceptable salt and adjuvant, which perform effect on inhibition of osteoclast genesis, so as to prevent the osteoporosis effectively. In summary, the present invention provides biphenyl benzamide-derived derivatives with high salt-resistance and provides a method for increasing the salt resistance of antibacterial peptide to solve the problem that the salt-resistance of antibacterial peptide is low. 

What is claimed is:
 1. A biphenyl benzamide-derived derivatives, which structure is selected from formula I:

wherein said R1, R2, R3 and R4 of the formula I is selected from the group consisting of H, halogen, CF3, CN, CH and OCH3.
 2. A pharmaceutical composition of biphenyl benzamide-derived derivatives, which comprises: (a) a structure is selected from formula II

(b) a pharmaceutical acceptable salt and carrier of the biphenyl benzamide-derived derivatives. wherein said R1, R2, R3 and R4 of the formula I is selected from the group consisting of H, halogen, CF3, CN, CH and OCH3.
 3. The pharmaceutical composition of claim 2, wherein the pharmaceutical acceptable carrier is excipient, diluents, thickeners, filler, binder, disintegrants, lubricant, oil or non-oil base, surfactant, suspending agent, gelling agent, adjuvant, anti-corrosive agent, anti-oxidant, stabilizer, coloring agent or flavor.
 4. The pharmaceutical composition of claim 2, wherein the salt is physiological acceptable salt of inorganic acid, inorganic base, organic acid or organic base.
 5. The pharmaceutical composition of claim 2, wherein said composition is powder, granule, liquid, gel or cream.
 6. The pharmaceutical composition of claim 2 wherein said composition is administrated through oral, transdermal, injection, or inhalational manner.
 7. A method for synthesis of benzamide-derived derivatives, wherein a compound of formula I is synthesized by difunisal:


8. The method of claim 7, wherein the compound of formula I is synthesized by amine, tetrahydrofuran and an intermediate compound which is synthesized by difunisal, tetrahydrofuran and thionyl chloride.
 9. An anti-inflammatory agent of biphenyl benzamide-derived derivatives for treating osteoporosis or osteoarthritis, which comprises: a structure is formula I.

wherein said R1, R2, R3 and R4 of the formula selected from the group consisting of H, halogen, CF3, CN, CH and OCH3. 